Effects of bisphenol A exposure during cardiac cell differentiation

Heart development requires a precise temporal regulation of gene expression in cardiomyoblasts. Therefore, the transcriptional changes differentiating cells can lead to congenital heart diseases. Although genetic mutations underlie most these alterations, exposure environmental contaminants, such as bisphenol A (BPA), has been recently considered risk factor well. In this study we investigated genotoxic and epigenotoxic effects BPA throughout cardiomyocyte differentiation. H9c2 (rat myoblasts) were exposed 10 30 ?M before during last two days cardiac-driven Then, have analysed phenotypic molecular modifications (at transcriptional, epigenetic level). The results showed that treated myoblasts developed skeletal muscle cell-like phenotype. induced by genes codifying proteins involved differentiation function depend on window BPA. repressed transcription factors (Hand2 Gata4), whereas reduced cardiac-specific (Tnnt2, Myom2, Sln, Atp2a1). Additionally, significant observed regarding DNA damage histone acetylation levels after periods exposure: toxicant percentage repair foci (formed co-localization ?H2AX 53BP1) increased dose-dependent manner, treatment with triggered decrease marks H3K9ac H3K27ac. Our vitro reveal seriously interferes process differentiation, which could be related reported vivo cardiogenesis.